Image processing was carried out with the image analysis software AMBA on the basis of the standard karyotyping software KARYOTYP that was modified and extended for the CGH purpose. The software was installed on a 90 MHz Pentium PC, running Microsoft Windows (Microsoft Corp., Redmond, Washington, U.S.A.).
Please contact Iver.Petersen@charite.de or Karsten.Schluens@charite.de for further information.The program comprises
the following steps:
1) image segmentation using the DAPI image;
2) correction of optical shift;
3) computation of the fluorescence ratio images between FITC and
TRITC images;
4) karyotyping;
5) determination of chromosome axis;
6) stretching of chromosomes and calculation of profiles;
7) evaluation.
Step 7
(evaluation) can be performed via the Internet by using your web browser.
Have a look at
the CGH Online Tumor Database.